THE MITOTIC CYCLE IN AN AMPHIPLOID (TRITICALE) AND ITS PARENTAL SPECIES

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Abstract
The durations of the mitotic cycle and its phases were determined by 3H-thymidine labelling of root meristematic cells of a strain of a wheat-rye amphiploid (triticale), and its parents Triticum turgidum L. var durum 'Stewart' and Secale cereale L. 'Prolific'.The duration, in hours, of the various phases of the mitotic cycle were:[Formula: see text]Feulgen microspectrophotometry showed triticale to have 15% less DNA per 4C nucleus than the sum total of its parents Prolific and Stewart whose DNA contents were in the ratio of 2:3, indicating that the duration of the S period is independent of DNA content per nucleus. The rate of DNA synthesis, measured as number of silver grains over interphase nuclei, was correlated with the total area of the nuclear membrane.