Amino Acid Incorporation into Protein of Diabetic Rat Liver Slices After Acute Insulin Deprivation.

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Abstract
Incorporation of leucine-1-C14 into protein of liver slices decreased progressively up to 48 hours after insulin withdrawal from alloxan-diabetic rat liver donors. Insulin (0.01 unit/ml) and glucose (200 mg/100 ml) added together to the Krebs-bicarbonate medium stimulated this incorporation at 24 hours after insulin withdrawal from the liver donors, smaller but statistically non-significant stimulation was obtained at 12 and 48 hours. Neither insulin nor glucose alone gave a significant stimulation in any diabetic group. Data previously had also shown that insulin added in vitro can stimulate incorporation of leucine into protein in livers from mildly, but not severely, insulin-deficient rats.