Monolithic Silica-Based Capillary Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry for Plant Metabolomics
- 28 October 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 75 (23) , 6737-6740
- https://doi.org/10.1021/ac034716z
Abstract
Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.Keywords
This publication has 21 references indexed in Scilit:
- Strategies for the Assessment of Matrix Effect in Quantitative Bioanalytical Methods Based on HPLC−MS/MSAnalytical Chemistry, 2003
- High-throughput classification of yeast mutants for functional genomics using metabolic footprintingNature Biotechnology, 2003
- Hydrophilic Interaction Liquid Chromatography Coupled to Electrospray Mass Spectrometry of Small Polar Compounds in Food AnalysisAnalytical Chemistry, 2003
- Profiling Membrane Lipids in Plant Stress ResponsesJournal of Biological Chemistry, 2002
- Analysis of Highly Polar Compounds of Plant Origin: Combination of Hydrophilic Interaction Chromatography and Electrospray Ion Trap Mass SpectrometryAnalytical Biochemistry, 2002
- Effect of eluent on the ionization efficiency of flavonoids by ion spray, atmospheric pressure chemical ionization, and atmospheric pressure photoionization mass spectrometryJournal of Mass Spectrometry, 2001
- Discrimination of Aerobic Endospore-forming Bacteria via Electrospray-Ionization Mass Spectrometry of Whole Cell SuspensionsAnalytical Chemistry, 2001
- Peer Reviewed: Monolithic LC ColumnsPublished by American Chemical Society (ACS) ,2001
- An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry dataJournal of the American Society for Mass Spectrometry, 1999
- Liquid chromatography–electrospray mass spectrometry of β-carotene and xanthophyllsJournal of Chromatography A, 1999