Ultrastructure and Chemical Analysis of the Cell Wall of Pythium debaryanum1

Publisher Website
Google Scholar
Abstract
The ultrastructure and component polysaccharides of the cell wall of P. debaryanum IFO-5919 were investigated. From results obtained by means of acid, alkali, Schweitzer reagent and .beta.-1, 3-glucanase treatments and EM, it was concluded that the acid-extracted fraction was a 1,3-linked branched glucan; the alkali-extracted fraction was a mixture of 1,3-, 1,6- and 1,3,6-linked highly branched 2 glucans; the Schweitzer reagent-extracted fraction was a .beta.-1, 4-linked glucan; the cell wall was constructed from 2 types of cellulosic microfibrils, as a frame and as a finer network, and amorphous .beta.-1, 3-glucan including .beta.-1, 6-linkage; and cellulosic microfibrils were covered by matrix material consisting of a mixture of amorphous .beta.-1, 3-linked and .beta.-1, 6-linked branching glucans.