• 1 January 1979
    • journal article
    • research article
    • Vol. 93  (3) , 499-505
Abstract
Human collagens were isolated from kidney, lung, skin, aorta, cartilage and placenta. Five different types were obtained, including 2 new molecular species, 1 characteristic of basement membranes, or type IV collagen, and the other the recently described A-B collagen derived from fetal membranes. All the collagens were purified and separated by combination of heat-gelation fractionation and salt fractionation. In neutral solution at 37.degree. C, neither type IV nor type A-B collagen elicited [human] platelet aggregation or 14C-serotonin release. Preincubation of platelets with both types IV and A-B collagen did not inhibit aggregation upon subsequent addition of collagen types I, II or III.