KINETICS OF CYANATE-HEMOGLOBIN REACTION IN WHOLE-BLOOD

  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (5) , 978-986
Abstract
The mechanism of the cyanate-Hb reaction was 2nd order, irreversible and bimolecular. The influence of the temperature, pH, PO2 [O2 tension] and PCO2 [CO2 tension] on the reaction rate constant was examined. A temperature change from 37.degree. to 42.degree. C, resulted in a 50% increase in the rate constant. The rate constant increased in proportion to the pH decrease, and deoxygenated whole blood [human] was carbamylated approximately twice as fast as oxygenated blood. CO2 pressures had no influence on rates of carbamylation when a constant pH was maintained. A maximum rate constant of 3.7 M-1 min-1 was obtained with conditions which were compatible with the red blood cells. This knowledge of the reaction mechanism and the influence of important system parameters on the reaction rate constant may be applied to the development of an extracorporeal system for the treatment of sickle cell anemia.

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