Apoptotic hepatocytes become insoluble in detergents and chaotropic agents as a result of transglutaminase action
- 13 March 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 245 (1-2) , 150-154
- https://doi.org/10.1016/0014-5793(89)80210-8
Abstract
Physiological deletion of cells ensues programmed death which involves formation of apoptotic bodies with fragmented DNA. Here we report that apoptotic hepatocytes are insoluble in detergents, urea, guanidine hydrochloride, reducing agents and thereby can be isolated from rat liver following collagenase treatment. They are wrinkled, spherical structures similar to cornified envelopes of epidermis by phase-contrast microscopy and show irregular, globular morphology by scanning-electron microscopy. Part of their DNA content is cleaved into nueleosomal and oligonucleosomal fragments. Their insolubility, like that of the cornified envelope, is evoked by ε-(γ-glutamyl)lysine and N 1,N 8-bis(γ-glutamyl)spermidine protein cross-linking bonds formed by transglutaminase.Keywords
This publication has 20 references indexed in Scilit:
- High-performance liquid chromatographic method for the determination of ε-(γ-glutamyl)lysine and mono- and bis-γ-glutamyl derivatives of putrescine and spermidineJournal of Chromatography A, 1988
- Induction and activation of tissue transglutaminase during programmed cell deathFEBS Letters, 1987
- Quantitative histological and histochemical studies on the occurrence and stages of controlled cell death (apoptosis) during regression of rat liver hyperplasiaVirchows Archiv B Cell Pathology Including Molecular Pathology, 1986
- Rapid analysis of amino acids using pre-column derivatizationJournal of Chromatography B: Biomedical Sciences and Applications, 1984
- Controlled death (apoptosis) of normal and putative preneoplastic cells in rat liver following withdrawal of tumor promotersCarcinogenesis: Integrative Cancer Research, 1984
- Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesisThe Journal of Pathology, 1984
- Significance of Cross-Linking of α2-Plasmin Inhibitor to Fibrin in Inhibition of Fibrinolysis and in HemostasisJournal of Clinical Investigation, 1982
- TransglutaminasesAnnual Review of Biochemistry, 1980
- Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activationNature, 1980
- The cornified envelope of terminally differentiated human epidermal keratinocytes consists of cross-linked proteinCell, 1977