Abstract
The activities of polyphenol oxidase and peroxidase per unit area of attached tobacco leaves increased as the leaves expanded, and reached a stable level in the mature leaves. After the onset of senescence the enzyme activities fell rapidly, but at a later stage they showed a small rise. Enzyme activities in tissues taken from non-senescent leaves increased during incubation at 20 °C. These increases were sensitive to inhibitors of protein synthesis. Enzyme activities in tissues taken from leaves in early senescence increased during incubation at 35 °C but not at 20 °C. These increases were very largely insensitive to inhibitors of protein synthesis and were not apparently related to de novo protein synthesis. There were no increases in enzyme activities in tissues taken from leaves in late senescence and incubated at 35 °C or 20 °C.

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