Colorimetric Method for Estimation of Proteose-Peptone in Milk
Open Access
- 1 June 1965
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 48 (6) , 674-676
- https://doi.org/10.3168/jds.s0022-0302(65)88320-5
Abstract
The milk serum obtained after the removal of casein and whey proteins by adjusting the pH of heated milk to 4.5, was used for estimating proteose-peptone. Color was developed with such serum before and after treatment with trichloroacetic-acid with Folin''s phenol reagent and read at 750 m[mu]. Absorption maximum, color-concentration proportionality, added recovery and comparison of N value with colorimetric value for proteose-peptone satisfied the requirements for colorimetric determination. Advantages offered by the method are simplicity and sensitivity of the reaction. Using this method, average values of 220.9 [plus or minus] 9.9 mg and 172.1 [plus or minus] 7.6 mg of proteose-peptone/100 ml of cow and buffalo milk were obtained, respectively.Keywords
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