Extracellular 2‐chloroadenosine and ATP stimulate volume‐sensitive Cl− current and calcium mobilization in human tracheal 9HTEo− cells

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Abstract
The perforated‐patch whole‐cell technique was used to record membrane currents in epithelial cells (9HTEo−) obtained from the human tracheal epithelium. Extracellular application of 2‐chloroadenosine and ATP (0.01–100 μM) caused activation of Cl− currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca2+ or by the addition of the A2 adenosine receptor antagonist 3,7‐dimethyl‐1‐propargylxanthine (DMPX). Fluorimetric measurements with fura‐2 reveal that 2‐chloroadenosine and ATP elicited both a Ca2+ influx through the plasma membrane and a release from intracellular stores.