Effect of riboflavin-butyrate on cardiac glutathione reductase affected by adriamycin.

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Abstract
Male Wistar rats received i.p. injections of adriamycin (4 mg/kg body wt/day) and/or riboflavin-butyrate (20 mg/kg body wt/day) for 6 consecutive days. Cardiac mitochondria were then prepared for this experiment. The combined use of riboflavin butyrate with adriamycin was evaluated for reduction of lipid peroxide formation in rat cardiac mitochondria. To find the mechanism of the effect of riboflavin-butyrate, the glutathione peroxidase-glutathione reductase system was examined. Adriamycin reduced the glutathione reductase activity in rat cardiac mitochondria, but did not affect the glutathione peroxidase activity. The mitochondrial content of flavin adenine dinucleotide, a prosthetic group of glutathione reductase, was greatly reduced and apoprotein of glutathione reductase also decreased. The administration of riboflavin-butyrate with adriamycin increased flavin adenine dinucleotide and glutathione reductase activity. Exogenous administration of riboflavin-butyrate is evidently capable of reducing lipid peroxide by enzymatic detoxification through glutathione reductase and non-enzymatic detoxification due to direct reaction with lipid peroxide.