31P NMR investigations on free and enzyme bound thiamine pyrophosphate
- 20 June 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 233 (2) , 379-382
- https://doi.org/10.1016/0014-5793(88)80465-4
Abstract
Pyruvate decar☐ylase (PDC) contains thiamine pyrophosphate (TPP) and Mg2+ as cofactors. 31P NMR studies with PDC in the presence of added Mn2+ reveal the pyrophosphate moiety of TPP to be a nonaccessible area for the external Mn2+ and thus proving the Mg‐P‐complex (taking part in the binding of the coenzyme to the protein) to be a nonaccessible area for the medium. Glyoxylic acid, acting as an inhibitor of PDC by forming a noncleavable bond with the catalytic center of TPP causes a steric immobilization of the coenzyme indicated by a line broadening of the pyrophosphate moiety.Keywords
This publication has 10 references indexed in Scilit:
- 31P nuclear magnetic resonance of mitochondrial aspartate aminotransferase. The effects of solution pH and ligand binding.Journal of Biological Chemistry, 1982
- Phosphorus-31 nuclear magnetic resonance study of the thiamine diphosphate-magnesium interactionArchives of Biochemistry and Biophysics, 1981
- Glucose oxidase contains a disubstituted phosphorus residue. Phosphorus-31 nuclear magnetic resonance studies of the flavin and nonflavin phosphate residuesBiochemistry, 1981
- Hydroxyl‐Ion‐Induced Subunit Dissociation of Yeast Cytoplasmic Pyruvate DecarboxylaseEuropean Journal of Biochemistry, 1980
- Phosphorus-31 nuclear magnetic resonance study of D-serine dehydratase: pyridoxal phosphate binding siteBiochemistry, 1979
- Glyoxylic acid as an active site marker of yeast pyruvate decar☐ylaseFEBS Letters, 1976
- Theoretical calculations on thiamine and related compounds. II. Conformational analysis and electronic properties of 2-(.alpha.-hydroxyethyl)thiamineJournal of the American Chemical Society, 1976
- 31P Nuclear‐Magnetic‐Resonance Studies of Pyridoxal and Pyridoxamine PhosphatesEuropean Journal of Biochemistry, 1975
- A new procedure to prepare highly purified and crystallized yeast pyruvate decarboxylaseFEBS Letters, 1974
- [19] Yeast pyruvate decar☐ylase (2-Oxoacid car☐y-lyase, EC 4.1.1.1) assay of thiamine pyrophosphatePublished by Elsevier ,1970