Novel method of biotyping Haemophilus influenzae that uses API 20e

Abstract
H. influenzae isolates (100) from various body (human) sites were biotyped by conventional methods and by the API 20E system. By using a hemin- and a NAD enriched saline solution as the inoculating fluid for the API 20E, a 100% correlation of results was obtained between the 2 methods. Of the blood and CSF isolates, 90% were biotype I. Biotype II was the predominant biotype encountered overall. No correlation was observed between .beta.-lactamase production and biotype. The API 20E is a reliable method and should prove useful for routine biotyping of H. influenzae in the clinical laboratory.

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