Molecular and Genomic Analysis of Genes Encoding Surface-Anchored Proteins from Clostridium difficile
Open Access
- 1 May 2001
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (5) , 3442-3446
- https://doi.org/10.1128/iai.69.5.3442-3446.2001
Abstract
The gene slpA , encoding the S-layer precursor protein in the virulent Clostridium difficile strains C253 and 79–685, was identified. The precursor protein carries a C-terminal highly conserved anchoring domain, similar to the one found in the Cwp66 adhesin (previously characterized in strain 79–685), an SLH domain, and a variable N-terminal domain mediating cell adherence. The genes encoding the S-layer precursor proteins and the Cwp66 adhesin are present in a genetic locus carrying 17 open reading frames, 11 of which encode a similar two-domain architecture, likely to include surface-anchored proteins.Keywords
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