BIOCHEMICAL VARIATION OF HUMAN IA LIKE ANTIGENS DETECTED WITH MONOCLONAL-ANTIBODIES

  • 1 January 1983
    • journal article
    • research article
    • Vol. 53  (1) , 41-50
Abstract
Four mouse monoclonal antibodies to human B cell surface determinants previously described as being directed against Ia like (MHC [major histocompatibility complex] class II) antigens, precipitate Ia .alpha. and .beta. chains. The antibodies were then used to analyze a range of cell types and a large number of lymphoblastoid and lymphoma cell lines. Ia antigens could not be detected on peripheral blood T cells, cord endothelium or T cells lines but their presence was confirmed on activated T cells and peripheral blood non-T cells. There was both qualitative and quantitative variation of Ia like antigen expression on B cell lines, including an apparent genetic polymorphism in .alpha. chain structure unrelated to DR allotypes and a single instance of a .beta. chain of abnormally high MW.