Isolation of a Potyvirus from Declining Clones ofPopulus

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Abstract
A virus was transmitted mechanically to herbaceous indicator plants from 6 declining P. .times. euramericana clones, 2 declining P. tremuloides clones and 2 declining P. grandidentata clones. The virus induced local lesions on inoculated Chenopodium quinoa and both local lesions and systemic symptoms on Phaseolus vulgaris and Vigna unguiculata (cowpea). Usually < 10% of the herbaceous indicator plants inoculated with leaf sap from declining poplars became infected. When inoculated with leaf sap of infected cowpea, P. tremuloides seedlings developed chlorotic and necrotic leaf spot symptoms similar to those observed on declining poplars. The virus was transmitted back to cowpea from inoculated symptomatic but not asymptomatic seedlings of P. tremuloides. EM of 4 representative isolates revealed flexuous rod-shaped virus particles (with a modal length of 800-810 nm) in sap from cowpea leaves and associated potyvirus-induced inclusions in thin sections of cowpea leaf tissue. All 4 isolates had a thermal inactivation point between 58.degree. and 60.degree. C, a dilution end point of 10-4 in cowpea leaf sap, a longevity in vitro of 24 h at 22.degree. C, and identical host ranges. This virus is a member of subdivision I of the potyvirus group. It can be differentiated from other viruses in that group by particle length, host range and symptomatology.

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