Automated Enzymatic Method for the Determination of Ammonia: Application to Rumen Fluid, Gut Fluid, and Plasma
- 1 August 1979
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 38 (2) , 212-215
- https://doi.org/10.1128/aem.38.2.212-215.1979
Abstract
The enzymatic method for the determination of NH3 with glutamate dehydrogenase was adapted to the AutoAnalyzer. The automated method was applicable for NH3 measurement in sheep rumen samples, swine gut fluid samples and bovine plasma. Results were compared with those of the Conway diffusion method and the manual enzymatic method. The automated method allows 30 samples/h to be analyzed routinely. Values were comparable with those with the manual method, but lower than those with the Conway diffusion method.This publication has 3 references indexed in Scilit:
- An enzymic assay for ammonia in waste matterJournal of Agricultural and Food Chemistry, 1977
- Enzymatic determination of ammonia in blood plasmaClinica Chimica Acta; International Journal of Clinical Chemistry, 1972
- AN ENZYMATIC DETERMINATION OF AMMONIA IN BIOLOGICAL FLUIDS1965