STUDIES ON THE PRODUCTION OF GLYCOLIPIDE BY PSEUDOMONAS AERUGINOSA

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Abstract
Glycolipide was produced by a strain of P. aeruginosa on both a bactopeptone and a synthetic medium. Fructose as well as glycerol could act as carbon source for its synthesis, and atmospheric CO2 plays a role. A satisfactory assay was established, and its validity confirmed by the isotope dilution technique. The results of the assay are consistent with the structure previously proposed by other workers. Glycolipide formation appears to be a function of an aging bacterial population with its onset following the logarithmic phase of growth. Glucose, citrate, succinate, and acetate additions sharply reduce glycolipide production in the presence of glycerol. In the case of acetate, inhibition begins when 10% and is complete when 35% of the C source is furnished by acetate C. Both rate of formation and total accumulation are affected.