The Regulated Expression of Epstein--Barr Virus. III. Proteins Specified by EBV During the Lytic Cycle

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Abstract
Virus-induced or virus-specified proteins (30) were synthesized in Raji [human lymphoblastoid] cells after superinfection with Epstein-Barr virus (EBV) derived from P3HR1 [human lymphoblastoid] cells. Using a combination of pulse labeling, application of cycloheximide blocks at different times post-infection, treatment with amino acid analogs and inhibition of DNA synthesis it was shown that 3 groups of proteins appear in Raji cells after superinfection; the synthesis of the proteins in any one group appears to be coordinately regulated. The 6 virus-induced proteins synthesized immediately after release from an early cycloheximide block should include those proteins essential for the transition from EBNA [Epstein-Barr virus nuclear antigen] to EA [early antigen] synthesis. Using human sera with differing specificities for the various antigen groups 11 proteins were identified as being specifically precipitated by sera having high titers against the EBV-induced EA complex.