Fab2 FRAGMENTS FROM HLA XENOANTISERA SPECIFICALLY BLOCK CYTOLYSIS MEDIATED BY HLA‐A, B ALLOANTISERA

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Abstract
Fab2 fragments from antisera raised in rabbits with partially purified cellular and serum HLA antigens were tested for their ability to block the cytolytic activity of operationally specific HLA-A,B alloantisera. One Fab2 fragment preparation blocked the cytolytic activity of all the HLA-A,B alloantisera tested; the remaining 9 inhibited the lytic activity of alloantisera to certain HLA-A,B allospecificities, suggesting that these xenoantisera contain antibody to certain HLA-A,B allotype determinants or to closely associated structures. None of the xenoantisera contained significant amounts of antibodies to human .beta.2-microglobulin.