A Novel Nuclear Member of the Thioredoxin Superfamily
Open Access
- 1 November 1998
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 118 (3) , 987-996
- https://doi.org/10.1104/pp.118.3.987
Abstract
We describe the isolation and characterization of a cDNA encoding maize (Zea mays L.) nucleoredoxin (NRX), a novel nuclear protein that is a member of the thioredoxin (TRX) superfamily. NRX is composed of three TRX-like modules arranged as direct repeats of the classic TRX domain. The first and third modules contain the amino acid sequence WCPPC, which indicates the potential for TRX oxidoreductase activity, and insulin reduction assays indicate that at least the third module possesses TRX enzymatic activity. The carboxy terminus of NRX is a non-TRX module that possesses C residues in the proper sequence context to form a Zn finger. Immunolocalization preferentially to the nucleus within developing maize kernels suggests a potential for directed alteration of the reduction state of transcription factors as part of the events and pathways that regulate gene transcription.Keywords
This publication has 30 references indexed in Scilit:
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- REDOX REGULATION OF CELLULAR ACTIVATIONAnnual Review of Immunology, 1997
- The Miniature1 Seed Locus of Maize Encodes a Cell Wall Invertase Required for Normal Development of Endosperm and Maternal Cells in the Pedicel.Plant Cell, 1996
- The Miniature1 Seed Locus of Maize Encodes a Cell Wall Invertase Required for Normal Development of Endosperm and Maternal Cells in the PedicelPlant Cell, 1996
- Identifying and Characterizing a Structural Domain of Protein Disulfide IsomeraseBiochemistry, 1996
- Thioredoxin activity in the C terminus of Phalaris S proteinThe Plant Journal, 1995
- Protein disulphide isomerase: building bridges in protein foldingTrends in Biochemical Sciences, 1994
- The Viviparous-1 developmental gene of maize encodes a novel transcriptional activatorCell, 1991
- ARF-B2: A Protein Complex that Specifically Binds to Part of the Anaerobic Response Element of Maize Adh 1Plant Physiology, 1990
- Immunoelectron microscopic localization of glutaredoxin and thioredoxin inEscherichia colicellsFEBS Letters, 1981