Isolierung und NMR-spektroskopische Charakterisierung sialinsäurehaltiger Verbindungen aus dem Hämofiltrat chronisch urämischer Patienten

1 January 1987

journal article
research article
Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler

Vol. 368 (1) , 647-658
https://doi.org/10.1515/bchm3.1987.368.1.647

Abstract

Eight sialyloligosaccharides have been isolated from the hemofiltrate of a patient with end stage renal disease using reverse osmosis gel filtration, ion-exchange and high-performance liquid chromatography. The structures were predominantly elucidated by one- and two-dimensional 1H- and 13C-NMR spectroscopy: 1 NeuAc.alpha.2-3Gal.beta.1-4Glc; 2 NeuAc.alpha.2-6Gal.beta.1-4Glc; 3 NeuAc.alpha.2-3Gal.beta.1-4GlcNAc; 4 NeuAc-.alpha.2-6Gal.beta.1-4GlcNAc; 5 NeuAc.alpha.2-3Gal.beta.1-4-GlcNAc.alpha.1-P; 6 NeuAc.alpha.2-6Gal.beta.1-4GlcNAc.alpha.1-P; 7 NeuAc.alpha.2-3Gal.beta.1-3GalNAc.alpha.1-P; 8 NeuAc.alpha.2-8NeuAc. While compounds 1-7 are also components of normal human urine, di-N-acetyl-D-neuraminic acid (8) could be isolated for the first time from biological material. The origin and possible clinical relevance of these compounds have to be proved in further investigations.

This publication has 0 references indexed in Scilit: