Analysis of Allergen Components in Grass Pollen Extracts using Immunoblotting
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 79 (4) , 434-440
- https://doi.org/10.1159/000234014
Abstract
Sera of 60 allergic persons and 20 healthy RAST-negative control persons were examined using immunoblotting to establish the presence of grass pollen-specific antibodies. These antibodies were depicted by an enzymatic, immunoglobulin class-specific indicator system. Seven bands of the timothy pollen extract proved to be major allergens. One of the 4 grass pollen-specific monoclonal antibodies tested (Bo 1) recognized the 7 major allergen bands. The possibility of employing monoclonal antibodies for the isolation of allergens is discussed.Keywords
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