Gonadotropic Activity in Bovine Serum and Placental Tissue1

Publisher Website
Google Scholar
Abstract
The presence of a bovine pregnancy gonadotropin (BPG), with LH-like [luteinizing hormone like] activity, was measured using the ventral prostate weight response in immature hypophysecto-mized rats. Phosphate buffer extracts (0.1 molar, pH 7.4) of maternal and fetal cotyledons and matrenal and fetal intercotyledonary tissue taken at 3-5 months of pregnancy were fractionated by means of gel filtration (using Sephadex G-100) and bio-assayed with the ventral prostate test. Significant response was observed from fractions of these 4 regions of reproductive tissues. Studies on the behavior of labeled steroids on the column showed that steroids were separated from nonsteoid fractions. Significant response in the ventral prostate bio-assay indicative of LH like activity was observed in an ammonium sulfate extract (0.25 molar, pH 7.0) of fetal cotyledons but no activity was found in the same which had been heat denatured. Some LH-like activity was measured in certain fractions of maternal jugular and uterine blood and all fractions of fetal umbilical blood. The results are interpreted to establish the existence of a gonadotropic substance in bovine placental tissue.

This publication has 0 references indexed in Scilit: