Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization

Top Cited Papers
Publisher Website
Google Scholar
Abstract
To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed, validated, and used with a set of five 16S rRNA-targeted oligonucleotide probes. Application of the six probes to fecal samples from 27 human adults showed additivity of 70% of the total 16S rRNA detected by the bacterial domain probe. The Bacteroidesgroup-specific probe accounted for 37% ± 16% of the total rRNA, while the enteric group probe accounted for less than 1%.Clostridium leptum subgroup and Clostridium coccoides group-specific probes accounted for 16% ± 7% and 14% ± 6%, respectively, while Bifidobacterium andLactobacillus groups made up less than 2%.