Molecular species of marine animal lipid-I. Molecular species of fish muscle lecithin.

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Abstract
A satisfactory separation of the molecular species of fish muscle lecithin was made by modifying the lecithin into diglceride acetate for high performance reversed phase liquid chromatography (HPLC), The molecular species of each chromatographic peak was determined by fatty acid composition and by total acyl carbon number analysis subsequent to separation, using a thin layer chronatographic technique with silver nitrate impregnated silica ged plated (Ag+-TLC). After plotting the relative retention time (RRT) of each molecular species from soybean, egg yolk, chum salmon, big-eyed tuna, Alaska pollack, and carp muscle lecithins semilogarithmically against the total cayl carbon number of the number of total double bonds of each molecular species, a set of parallel straight oblique lines was obtained. Thus we can express the molecular species in matrix relation by giving a variable integer x for the acyl carbon number and a variable integery for the number of double bonds of each fatty acid in the molecular species. By using the correlations between the RRTs and the corresponding molecular species on semilogarithmic plots, it was conclude that an identification of molecular species from fish lipids could be done by RRTs from HPLC

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