The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signals.
- 15 August 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (16) , 7442-7446
- https://doi.org/10.1073/pnas.89.16.7442
Abstract
Agrobacterium tumefaciens causes neoplastic growth in plants by transferring a piece of DNA, called T-DNA, into the nucleus of the plant cell. The virulence protein VirD2 of A. tumefaciens is tightly linked to the T-DNA and is thought to direct it to the plant genome. Here we show that the VirD2 protein contains two nuclear localization signals that are functional both in yeast and in plant cells. One signal is located in the N-terminal part of the protein and resembles a single-cluster-type nuclear localization signal. The second signal is near the C terminus and is a bipartite-type nuclear localization signal. The involvement of these sequences in the entry of the T-DNA into the nucleus is discussed.Keywords
This publication has 33 references indexed in Scilit:
- Mutational analysis of Agrobacterium tumefaciens virD2: tyrosine 29 is essential for endonuclease activityJournal of Bacteriology, 1992
- Illegitimate recombination in plants: a model for T-DNA integration.Genes & Development, 1991
- How proteins enter the nucleusCell, 1991
- Monocot regulatory protein Opaque-2 is localized in the nucleus of maize endosperm and transformed tobacco plants.Plant Cell, 1991
- A bacterial peptide acting as a plant nuclear targeting signal: the amino-terminal portion of Agrobacterium VirD2 protein directs a beta-galactosidase fusion protein into tobacco nuclei.Proceedings of the National Academy of Sciences, 1990
- The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signalCell, 1990
- VirD2gene product from the nopaline plasmid pTiC58 has at least two activities required for virulenceNucleic Acids Research, 1990
- Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation.Proceedings of the National Academy of Sciences, 1989
- Sequence requirements for synthetic peptide-mediated translocation to the nucleus.Molecular and Cellular Biology, 1989
- The Agrobacterium tumefaciens virE2 gene product is a single-stranded-DNA-binding protein that associates with T-DNAJournal of Bacteriology, 1988