The Negative Staining of Bacteria

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Abstract
The author does not propose new methods but calls attention to the value of Burri's India ink technic and Fischer's use of nigrosin for the negative staining of bacteria. The latter is particularly useful. The technic is as follows: Boil 10 g. nigrosin in 100 cc. water about 30 minutes. Filter several times thru the same filter paper, adding 10 drops of formalin before the last filtration as a preservative. Place a small loopful of this solution on a clean slide and add the bacteria with a needle or loop. After mixing spread the mixture a little irregularly on the slide and dry either at room temperature or slowly over a low flame. The preparation may be examined in oil immersion without the use of a cover slip.

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