STORAGE OF PLATELET CONCENTRATES USING ION-EXCHANGE RESIN CHARGED WITH DIBASIC PHOSPHATE

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 58  (3) , 537-543
Abstract
Platelet [human] concentrates were prepared at twice the normal concentration and stored at room temperature for 7 days in either standard bags (controls) or bags to which 1 or 2 g of Amberlite resin beads charged with dibasic phosphate had been added. The resin beads served as a buffer system by providing a slow release form of phosphate ions as well as by binding CO2 produced during platelet metabolism. Control platelets demonstrated rapid falls in pH, ATP content, morphology score and thrombin-induced nucleotide release after 24 h of storage with a fall in pH to less than 6.0 by day 3. Profound ultrastructural changes and a rise in p[partial pressure]O2, suggesting a loss of platelet viability, accompanied these changes. The resin-stored platelets remained near normal after 24 h of storage, with preservation of discoid morphology, 95% of ATP levels, excellent ultrastructural appearance and evidence of continued O2 consumption after 3 days of storage. Even after 7 days of storage, ATP levels remained greater than 50% of baseline and ultrastructurally intact platelets were seen. In the 1 g resin bags the pH remained at baseline levels (6.9-7.0), while there was a rise in pH in the 2 g resin bags. The beneficial effects of maintaining a higher pH during platelet storage are demonstrated and a new approach to studying the metabolic changes that occur during longer term storage is provided.