Production of Human Monoclonal Antibody in Mouse Ascites
- 1 January 1984
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 3 (2) , 195-199
- https://doi.org/10.1089/hyb.1984.3.195
Abstract
Human B-cell hybridomas were grown in nude mouse ascites. The growth of cells in ascites requires prior subcutaneous (SC) adaptation, but the amount of antibody harvested is 100-fold that produced in in vitro culture. The relative merits of in vitro versus in vivo production of human monoclonal antibodies are discussed.This publication has 3 references indexed in Scilit:
- Characterization of human hybridomas secreting antibody to tetanus toxoid.Proceedings of the National Academy of Sciences, 1983
- Routine large-scale production of monoclonal antibodies in a protein-free culture mediumJournal of Immunological Methods, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970