Engineering the selectivity of aliphatic C–H bond oxidation catalysed by cytochrome P450cam
- 1 January 1996
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- No. 21,p. 2413-2414
- https://doi.org/10.1039/cc9960002413
Abstract
The regioselectivity of the catalytic hydroxylation of phenylcyclohexane 1 by cytochrome P450cam can be altered dramatically by site-directed mutagenesis: the Y96F single site mutant gives 81%cis-3-phenylcyclohexanol 2, with 22% enantiomeric excess (ee), while the Y96F–V247A double mutant gives 97% 2 with 42% ee and the Y96F–V247L double mutant gives 83%trans-4-phenylcyclohexanol 4.Keywords
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