CD22-mediated stimulation of T cells regulates T-cell receptor/CD3-induced signaling.
- 1 November 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (21) , 10242-10246
- https://doi.org/10.1073/pnas.89.21.10242
Abstract
Interaction between B lymphocytes and other cell types is mediated in part by the B-cell adhesion molecule CD22. Recent work has suggested one of the T-cell ligands of B cells to be CD45RO, an isoform of the receptor-linked phosphotyrosine phosphatase CD45. Here we demonstrate direct interaction between CD22 and several isoforms of CD45, including CD45RO, and propose that the interaction may participate in regulation of lymphocyte signaling. Cross-linking of CD3 and CD22 T-cell ligands with anti-CD3 antibody and soluble CD22 is shown to block anti-CD3-induced intracellular calcium increase and to inhibit tyrosine phosphorylation of phospholipase C gamma 1. These effects are consistent with those observed upon coligation of CD3 and CD45 with antibody, providing support to the possibility that ligand-mediated stimulation of CD45 may result in modulation of substrate phosphorylation and lymphocyte activation.Keywords
This publication has 46 references indexed in Scilit:
- CD45. A prototype for transmembrane protein tyrosine phosphatases.Journal of Biological Chemistry, 1991
- CD62/P-selectin recognition of myeloid and tumor cell sulfatidesCell, 1991
- The B lymphocyte adhesion molecule CD22 interacts with leukocyte common antigen CD45RO on T cells and α2–6 sialyltransferase, CD75, on B cellsCell, 1991
- PLC gamma 1, a possible mediator of T cell receptor function.Journal of Biological Chemistry, 1991
- Functional activation of the T-cell antigen receptor induces tyrosine phosphorylation of phospholipase C-gamma 1.Proceedings of the National Academy of Sciences, 1991
- CD3 stimulation causes phosphorylation of phospholipase C-gamma 1 on serine and tyrosine residues in a human T-cell line.Proceedings of the National Academy of Sciences, 1991
- The B-cell surface protein CD72/Lyb-2 is the ligand for CDSNature, 1991
- The specific direct interaction of helper T cells and antigen-presenting B cells.Proceedings of the National Academy of Sciences, 1986
- Activators of protein kinase C down-regulate and phosphorylate the T3/T-cell antigen receptor complex of human T lymphocytes.Proceedings of the National Academy of Sciences, 1985
- Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule.Proceedings of the National Academy of Sciences, 1983