Hyaluronate Synthesis by Synovial Villi in Organ Culture

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Abstract
Individual canine synovial villi were used to establish short‐term synovial organ cultures. These villi incorporated 3H‐glucosamine into highly‐polymerized 3H‐hyaluronic acid (3H‐HA), which was the only 3H‐glycosaminoglycan identified in the culture medium. Some 3H‐HA, and larger amounts of other 3H‐glycosaminoglycans, were recovered from cultured tissues. Culture medium 3H‐HA content was proportional to the surface area of cultured villi. Organ cultures of nonvillous synovium were compared with villi; nonvillous cultures synthesized less 3H‐HA per mm2 of their synovial intimal surface than villi. These cultures complement cell culture techniques for in vitro studies of synovial lining cell function.