Localization and DNA sequence analysis of theCgene of bacteriophage Mu, the positive regulator of Mu late transcription
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (12) , 4881-4897
- https://doi.org/10.1093/nar/14.12.4881
Abstract
The C gene of bacteriophage Mu, required for transcription of the phage late genes, was localized by construction and analysis of a series of deleted derivatives of pKN50, a plasmid containing a 9.4 kb Mu DNA fragment which complements Mu C amber mutant phages for growth. One such deleted derivative, pWM10, containing only 0.5 kb of Mu DNA, complements C amber phages and transactivates the mom gene, one of the Mu late genes dependent on C for activation. The DNA sequence of the 0.5 kb fragment predicts a single long open reading frame coding for a 140 amino acid protein. Sequence analysis of DNA containing a C amber mutation located the base change to the second codon of this reading frame. Generation of a frameshift mutation by filling in a BglII site spanning codon 114 of this reading frame resulted in the loss of C complementation and transactivation activity. These results indicate that this open reading frame encodes the Mu C gene product. Comparison of the predicted amino acid sequence of the C protein with those of other transcriptional regulatory proteins revealed some similarity to a region highly conserved among bacterial sigma factors.Keywords
This publication has 45 references indexed in Scilit:
- Two functional domains conserved in major and alternate bacterial sigma factorsFEBS Letters, 1985
- Bacteriophage SPO1 genes 33 and 34Journal of Molecular Biology, 1984
- POSITIVE CONTROL OF TRANSCRIPTION INITIATION IN BACTERIAAnnual Review of Genetics, 1984
- A developmental gene product of Bacillus subtilis homologous to the sigma factor of Escherichia coliNature, 1984
- Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factorCell, 1984
- The operen that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12Cell, 1983
- Structural polypeptides and products of late genes of bacteriophage Mu: Characterization and functional aspectsJournal of Molecular Biology, 1981
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979
- Amino Acid Difference Formula to Help Explain Protein EvolutionScience, 1974
- In vitro Synthesis of Bacteriophage LysozymeNature, 1967