The Location of the Poly(C) Tract in the RNA of Foot-and-Mouth Disease Virus

Abstract
Fragments of foot-and-mouth disease virus RNA of decreasing size, containing the 3'' poly(A) sequence were prepared by alkali treatment and sucrose gradient centrifugation followed by oligo(dT)-cellulose affinity chromatography. Polyacrylamide gel electrophoresis of the RNase T1 resistant oligonucleotides from these polyadenylated fragments enabled the location of the position of some of the longer oligonucleotides on the RNA. The poly(C) tract was near the 5'' end of the RNA. The possible function of the poly(C) tract is discussed in the light of these findings.

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