Direct electrochemical characterization of the interaction between haemoglobin and nitric oxide
- 7 September 2000
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Physical Chemistry Chemical Physics
- Vol. 2 (19) , 4409-4413
- https://doi.org/10.1039/b005527l
Abstract
Direct electrochemistry was carried out to investigate the interaction between haemoglobin (Hb) and nitric oxide (NO), employing a Hb–DNA film modified electrode. DNA was used as an electrochemical “promoter” for the direct electron transfer of Hb at the electrode surface. Aside from a pair of peaks corresponding to the redox reaction of Hb, two additional peaks were observed in a solution containing NO. The pre-peak, located at −0.070 V s. SCE, was proposed to come from the chemical redox reaction, i.e., NO-induced oxidation of Hb in the film, coupled electron transfer process of Hb. The other peak, at −0.684 V s. SCE, was assigned to the reduction of heme-ligated NO. These phenomena were proposed to be related to the electron transfer and binding process between Hb and NO.Keywords
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