Étude de l'action de sels de cations monovalents sur les particules ribonucléoprotéiques de cerveau de rat

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Abstract
The dissociation of the nuclear particles by salts has been studied in order to throw some light upon their structure.The density of nuclear mono‐ and polyparticles in CsCl was progressively increased as a result of the action of increasing KCl concentrations (from 0.4 to 1.0 M). The results indicate that proteins are progressively released from the particles and are not consistent with the existence of an “informofer” stable at high salt concentration.Polyparticles of high sedimentation coefficient were more resistant to the action of salts than smaller ones. Respectively 69, 35 and 22% of 60‐S, 110‐S and 170‐S particles (density in CsCl: 1.40 g/ml) were dissociated with 1 M KCl into complexes of density (in CsCl) higher than 1.60 g/ml. A further increase in salt concentration or in time of contact, substitution of K+ by Na+ or Li+ ions or the replacement of Mg2+ by EDTA had little effect on the dissociation of 110–170‐S polyparticles. Polyparticles were more easily dissociated by salts when they were first treated with RNAase at very low concentrations, leading to only a few cuts of the polynucleotide chains but not to the hydrolysis of poly‐ into monoparticles. Together the results suggest a compact configuration for the polyparticles.

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