Improvement in Callus Growth and Plantlet Formation in Creeping Bentgrass1

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Abstract
Optimizing callus growth and plantlet formation from somatic callus is advantageous in the recovery of desirable variants using in vitro cell selection methods. Using 24‐week‐old ‘Penncross’ creeping bentgrass (‘Agrostis palustris Huds‘.) stock callus (SC), callus growth and plantlet formation were evaluated from plated SC previously cultured in suspension for 12 and 24 days and SC not previously cultured in suspension. In addition, callus growth and plantlet formation were evaluated from SC plated at aggregate sizes of 0.5‐ to 1.0‐mm and 0.25‐ to 0.5‐mm diam and aggregate densities of 1, 3, and 5 mg.50 mm−2. Callus growth was twofold greater from SC previously cultured in suspension than from SC not previously cultured in suspension. Plantlet formation was inhibited from SC previously cultured in suspension (−2 plating density. Aggregates 0.5‐ to 1.0‐mm and 0.25‐ to 0.5‐ mm diam had the greatest plantlet formation at 1 mg.50 mm−2 and 1 and 3 mg.50 mm−2 densities, respectively.