Isolation and Characterization of New Virulent ActinophageΦr5

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Abstract
A new virulent phage, Φr5, was isolated from soil on Streptomyces ribosidificus SF733. Electron microscopic observation showed that this phage belongs to group B of Bradley′s morphological classification. The molecular weight of Φr5 DNA was about 29 × 106. Φr5 DNA showed resistance to many restriction enzymes. Φr5 required Ca+ + for plaque formation and grew at 28°C on S. ribosidificus SF733, S. chartreusis SF1623, S.flavus, S. flavovirens, S. sulfonofaciens SF2103, S. sulfonofaciens SF2138 and S. sulfonofaciens SF2144. The restriction-modification of Φr5 by the strains susceptible to this phage was examined.