Nitrilotriacetic acid (NTA) induces aneuploidy in drosophila and mouse germ‐line cells
- 1 January 1988
- journal article
- research article
- Published by Wiley in Environmental Mutagenesis
- Vol. 12 (4) , 397-407
- https://doi.org/10.1002/em.2860120408
Abstract
The ability of nitrilotriacetic acid (NTA) to induce aneuploidy was studied in the germ line of both Drosophila and the mouse. The Free Inverted × Chromosomes (FIX) genetic system, adopting a brooding scheme, was used to detect induced aneuploidy in Drosophila, and a cytogenetic method based on chromosomal counting in secondary spermatocytes was used in the mouse. In Drosophila a highly significant (P < 0.001) increase of aneuploidies was produced by NTA (5 × 10−2 M), which was greater than that produced by colchicine (7.5 × 10−6 M) and 5‐fluorodeoxyuridine (10−4 M), which were used as positive controls. Brooding effects were observed with NTA, which produced a maximum induction of chromosomal gain in brood I, suggesting a possible stage‐specific action during meiosis. The ability of NTA (275 mg/kg body weight) to induce meiotic aneuploidy (hyperhaploidy) also was confirmed in the mouse (P < 0.001), where all the aneuploidies detected were attributable to treatment of the metaphase I stage.Keywords
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