Overexpression of Acyl Carrier Protein-1 Alters Fatty Acid Composition of Leaf Tissue in Arabidopsis
- 1 September 2001
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 127 (1) , 222-229
- https://doi.org/10.1104/pp.127.1.222
Abstract
Acyl carrier protein (ACP) is a small (9 kD) acidic protein that is an essential cofactor in plant fatty acid biosynthesis. Most plants have several isoforms of ACP, some of which are expressed constitutively and others that appear to be more tissue specific. Although the critical role of ACP in fatty acid biosynthesis has been established, the role of the diverse number of isoforms has yet to be elucidated. We have generated transgenic Arabidopsis plants that express high levels of ACP-1, a seed-predominant ACP isoform, in leaf tissue under control of the cauliflower mosaic virus 35S promoter. Western and northern analysis of these plants demonstrate 3- to 8-fold increased expression of this isoform in leaf tissue, but no significant changes in seed. Analysis of the fatty acid composition of leaf tissue revealed that overexpression of ACP-1 in leaf tissue alters fatty acid composition. Significant decreases in levels of 16:3 were noted along with increases in 18:3. These findings represent the first in vivo report that overexpression of an ACP isoform results in changes in fatty acid composition in plants.Keywords
This publication has 43 references indexed in Scilit:
- Isoforms of acyl carrier protein involved in seed‐specific fatty acid synthesisThe Plant Journal, 1999
- A Special Acyl Carrier Protein for Transferring Long Hydroxylated Fatty Acids to Lipid A in RhizobiumJournal of Biological Chemistry, 1996
- The Characterization of a Mitochondrial Acyl Carrier Protein Isoform Isolated from Arabidopsis thalianaPlant Physiology, 1994
- Expression of Constitutive and Tissue-Specific Acyl Carrier Protein Isoforms in ArabidopsisPlant Physiology, 1992
- Expression of holo and apo forms of spinach acyl carrier protein-I in leaves of transgenic tobacco plants.Plant Cell, 1989
- Plastid‐localised seed acyl‐carrier protein of Brassica napus is encoded by a distinct, nuclear multigene familyEuropean Journal of Biochemistry, 1988
- Activity of Acyl Carrier Protein Isoforms in Reactions of Plant Fatty Acid MetabolismPlant Physiology, 1986
- Metabolism of Unsaturated Monogalactosyldiacylglycerol Molecular Species in Arabidopsis thaliana Reveals Different Sites and Substrates for Linolenic Acid SynthesisPlant Physiology, 1986
- Cellular Organization of Glycerolipid MetabolismAnnual Review of Plant Physiology, 1982
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959