Hemoglobin British Columbia [α2β2101(G3)Glu→Lys] a New Variant with High Oxygen Affinity
- 1 January 1976
- journal article
- Published by Taylor & Francis in Hemoglobin
- Vol. 1 (2) , 171-182
- https://doi.org/10.3109/03630267608991678
Abstract
Hemoglobin British Columbia was found in an East Indian living in Vancouver, British Columbia, Canada. Its structure was demonstrated to be alpha2beta2 101(G3)Glu replaced by Lys. It has a significant increase in oxygen affinity, decrease in heme-heme interaction, but normal Bohr effect. Unlike Hb Rush (beta101 Glu replaced by Glu), it is as stable as Hb A to heat and alcohol denaturation. By both cellulose acetate electrophoresis and chromatography the undissociated Hb British Columbia moves between Hb S and Hb A rather than behaving like Hb C. However, the dissociated abnormal beta chain behaves like beta C. The substitution is at the alpha2beta2 contact region. Except for a mild erythrocytosis, the propositus has normal hematological findings.Keywords
This publication has 18 references indexed in Scilit:
- Application of glyeinamidation to structural studies of hemoglobinAnalytical Biochemistry, 1976
- Identification of Hemoglobins by ElectrophoresisCRC Critical Reviews in Clinical Laboratory Sciences, 1974
- Studies on the function of abnormal hemoglobins I. An improved method for automatic measurement of the oxygen equilibrium curve of hemoglobinBiochimica et Biophysica Acta (BBA) - Protein Structure, 1970
- Automatic Peptide ChromatographyPublished by Wiley ,1970
- Abnormal human haemoglobins: Separation and characterization of the α and β chains by chromatography, and the determination of two new variants, Hb chesapeake and Hb J (Bangkok)Journal of Molecular Biology, 1966
- A New Method for the Preparation of α and β Subunits of Human HemoglobinPublished by Elsevier ,1965
- Studies on the heterogeneity of hemoglobinJournal of Chromatography A, 1965
- Structural Studies of Aminoethylated Hemoglobins by Automatic Peptide ChromatographyCold Spring Harbor Symposia on Quantitative Biology, 1964
- Estimation of Small Percentages of Fœtal HæmoglobinNature, 1959
- Observations on the Minor Basic Hemoglobin Component in the Blood of Normal Individuals and Patients with ThalassemiaJournal of Clinical Investigation, 1957