Apoptosis in Maternal Peripheral Blood during Pregnancy
- 15 December 2000
- journal article
- Published by S. Karger AG in Fetal Diagnosis and Therapy
- Vol. 16 (1) , 32-37
- https://doi.org/10.1159/000053877
Abstract
Objective: To investigate the mononuclear cell apoptosis rate during pregnancy. Materials and Methods: Apoptosis was quantitated by EtBr staining in whole peripheral blood samples of 135 women in different gestational weeks and 85 nonpregnant women used as controls. Apoptosis was also qualitated by TUNEL assay. Results: The apoptosis rate increased during pregnancy according to gestational age. In chromosomally abnormal fetuses apoptosis was 2.5-fold higher than that found in pregnancies with normal embryos matched for gestational age. FISH in TUNEL-positive cells using X, Y and 21 chromosome probes verified the fetal origin of part of the apoptotic population. Conclusion: Apoptosis is stimulated in maternal peripheral blood during pregnancy, possibly accounting partly for the presence of free fetal DNA in maternal serum. The increased apoptosis rate in pregnancies with chromosomally abnormal fetuses may have additional clinical importance.Keywords
This publication has 6 references indexed in Scilit:
- Second‐trimester maternal serum progesterone levels in Turner syndrome with and without hydrops and in trisomy 18Prenatal Diagnosis, 1999
- Rapid Clearance of Fetal DNA from Maternal PlasmaAmerican Journal of Human Genetics, 1999
- Fetal DNA in Maternal Plasma: The Plot Thickens and the Placental Barrier ThinsAmerican Journal of Human Genetics, 1998
- Presence of fetal DNA in maternal plasma and serumThe Lancet, 1997
- Microsatellite alterations in plasma DNA of small cell lung cancer patientsNature Medicine, 1996
- Apoptosis And Programmed Cell Death In ImmunityAnnual Review of Immunology, 1992