On the role of 17 alpha‐estradiol and 17 beta‐estradiol in the proliferation of MCF7 and T47D‐A11 human breast tumor cells

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Abstract
A comparative study of the proliferative effect of 17 beta‐estradiol and 17 alpha‐estradiol on human estrogen‐sensitive cell lines was performed. When using charcoal‐dextran stripped human female sera‐supplemented media the administration of the hormones, 17 alpha‐estradiol at 3 × 10−10M, and 17 beta‐estradiol at 3 × 10−11M, resulted in a ten‐fold increase in cell yield when compared with non‐estrogen supplemented controls after cells were grown for periods between 10 to 14 days. No significant metabolization of 17 alpha‐estradiol into 17 beta‐estradiol occurred as measured by the E2 levels in the supernatants of the cell culture flasks. Increased concentrations of 17 beta‐estradiol and 17 alpha‐estradiol added to the media bathing C7MCF7‐173 cells resulted in a triggering of a partially successful shut‐off effect; this phenomenon was not observed with T47D‐All cells. These results are compatible with predictions stemming from the indirect and direct negative working hypothesis for the regulation of cell proliferation.