MAPPING AND TRANSCRIPTIONAL PROPERTIES OF RT1 CLASS II REGION GENES

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Abstract
The class II region of major histocompatibility complex of the rat. Rattus norvegicus (RT1) consists of RT1.B, RT1.D, and RT1.H subregions. The gene order around the H subregion was determined as RT1.A—Hβ-Hα—B by RFLP analysis of naturally occurring intra-RT1 recombinant rats with HLA DP probes. A unique recombinant strain, LEJ, was found to have its recombinational site between Hβ and Hα (RT1.A uHβ uHα bBbDb). Northern analysis of class II mRNAs showed that transcripts of RT1. Dα, RT1.Dβ, and RT1.Bα shared identical sizes among various strains of rats, but RT1.Bβ mRNA showed allele-specific size heterogeneities. Northern hybridization with HLA DPα probes detected possible RTl.Hα transcripts. On the other hand, no clear signal of Hβ was observed. BDIX whose RT1.B products had not been identified was found to transcribe Bα and Bβ mRNAs.

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