Nucleotide sequence of reovirus genome segment S3, encoding non-structural protein sigma NS
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (18) , 6399-6408
- https://doi.org/10.1093/nar/11.18.6399
Abstract
This report desoribes the complete nuoleotide sequence of human reovirus (Dearing strain) genome segment S3. Previous studies indicated that this RNA encodes the major non-structural viral polypeptlde sigma NS, a protein that binds ssRNAs (Huisman & Joklik, Virology 70, 411–421, 1976) and has a poly(C)-dependent poly(G) polymerase aotivity (Gomatos et al., J. Virol, 39, 115–124, 1981). The genome segment consists of 1,198 nuoleotldes and possesses an open reading frame that extends 366 codons from the first AUG triplet (residues 28–30). There is no significant sequenoe homology between the plus strand of genome segment S3 and that of genome segment S2 determined previously (Cashdollar et al., PNAS 79, 7644–7648, 1982). However, S3 RNA has significant dyad symmetry and regions that can potentially hybridize (δG = −26 KCal/mole) with S2 RNA. From the predicted amlno acid sequence a possible secondary structure for sigma NS protein vas determined. Structural features of reovirus RNA and sigma NS are discussed in relation to their role(s) in viral genome assembly.Keywords
This publication has 21 references indexed in Scilit:
- Prediction of the Secondary Structure of Proteins from their Amino Acid SequencePublished by Wiley ,1979
- The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3Virology, 1978
- Characterization of ribosome-protected fragments from reovirus messenger RNA.Journal of Biological Chemistry, 1976
- The Reovirus Replicative CycleAnnual Review of Biochemistry, 1976
- Reovirus-coded polypeptides in infected cells: Isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectivelyVirology, 1976
- Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.Proceedings of the National Academy of Sciences, 1975
- Conformational studies of reovirus single-stranded RNAs synthesized in vitroBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Temperature-sensitive mutants of reovirus. IV. Evidence that anomalous electrophoretic migration behavior of certain double-stranded RNA hybrid species is mutant group-specificVirology, 1973
- Temperature-sensitive mutants of reovirusVirology, 1972
- Enhancement of Reovirus Infectivity by Extracellular Removal or Alteration of the Virus Capsid by Proteolytic EnzymesJournal of General Virology, 1970