Effects of Ryo-Kan-Kyomi-Sin-Ge-Nin-To Extract on Degranulation of and Histamine Release from Rat Mast Cells

Abstract
The effects of Ryo-kan-kyomi-sin-ge-nin-to (RKSG) extract, a medicinal agent traditionally used in China and Japan for treatment of asthma, on the degranulation of and histamine release from rat mast cells were studied. At a concentration of 5 mg/ml RKSG, degranulation of mast cells stimulated either by antigen (DNP-Ascaris) or compound 48/80 was markedly suppressed. At a concentration of 1-5 mg/ml RKSG, histamine release from mast cells due to application of either antigen or compound 48/80 was inhibited in a dose-dependent fashion. These results suggest that RKSG may be useful for the treatment of type I allergy-related diseases.

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