Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance
Open Access
- 15 January 2007
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 176 (2) , 223-230
- https://doi.org/10.1083/jcb.200606116
Abstract
Na(+),K(+) ATPase is an essential ion pump involved in regulating ionic concentrations within epithelial cells. The zebrafish heart and mind (had) mutation, which disrupts the alpha1B1 subunit of Na(+),K(+) ATPase, causes heart tube elongation defects and other developmental abnormalities that are reminiscent of several epithelial cell polarity mutants, including nagie oko (nok). We demonstrate genetic interactions between had and nok in maintaining Zonula occludens-1 (ZO-1)-positive junction belts within myocardial cells. Functional tests and pharmacological inhibition experiments demonstrate that Na(+),K(+) ATPase activity is positively regulated via an N-terminal phosphorylation site that is necessary for correct heart morphogenesis to occur, and that maintenance of ZO-1 junction belts requires ion pump activity. These findings suggest that the correct ionic balance of myocardial cells is essential for the maintenance of epithelial integrity during heart morphogenesis.Keywords
This publication has 36 references indexed in Scilit:
- Elucidation of megalin/LRP2-dependent endocytic transport processes in the larval zebrafish pronephrosJournal of Cell Science, 2006
- Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesisDevelopment, 2006
- The small heart Mutation Reveals Novel Roles of Na + /K + -ATPase in Maintaining Ventricular Cardiomyocyte Morphology and Viability in ZebrafishCirculation Research, 2004
- Tight Junction Protein Par6 Interacts with an Evolutionarily Conserved Region in the Amino Terminus of PALS1/StardustJournal of Biological Chemistry, 2004
- Germ‐line transmission of a myocardium‐specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafishDevelopmental Dynamics, 2003
- Mutations of Ser‐23 of the α1 subunit of the rat Na+/K+‐ATPase to negatively charged amino acid residues mimic the functional effect of PKC‐mediated phosphorylationFEBS Letters, 1999
- Regulatory Phosphorylation of the Na+/K+‐ATPase frommalian Kidneys and Xenopus Oocytes by Protein KinasesAnnals of the New York Academy of Sciences, 1997
- Stages of embryonic development of the zebrafishDevelopmental Dynamics, 1995
- Activation of protein kinase C by phorbol ester induces downregulation of the Na+/K+-ATPase in oocytes ofXenopus laevisThe Journal of Membrane Biology, 1990
- Site-directed mutagenesis of Asp-376, the catalytic phosphorylation site, and Lys-507, the putative ATP-binding site, of the α-subunit of Torpedo californicaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1990