A Modification of the Thiocholine Method for the Demonstration of Cholinesterases

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Abstract
A modification of the thiocholine technique for cholinesterases is described, in which mounted sections are dehydrated for 2 min in each ascending grade of alcohol before development of CuS by using alcoholic (NH4)2S. This reagent is prepared by first saturating half-concentrated (NH4)2OH with H2S; 3 ml of this is diluted with 22 ml of ethyl alcohol, then saturated with CuS. With these modifications better definition of sites of cholinesterase activity is achieved.