Observational and experimental studies of the meristem of leguminous plants. II. Differentiation of the pericycle in the root tips of Pisum sativum.

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Abstract
Available data concerning the differentiation of the pericycle in the primary root tip of P. sativum, cv. ''Alaska'', is summarized. The root tips from 3-4 day old seedlings germinated in a darkroom at 24.degree. C were fixed with Lillie''s buffered neutral formalin under green light. By applying a combination of Lillie''s formalin fixation together with methyl blue and/or Heidenhain''s hematoxylin and safranin, the initial symptom of triarch xylary procambium appeared in a procambial cylinder in the transverse section of 250 .mu. from the apical initials. Deeply stainable protoplasts of several layers of the pericyclic cells localized outside of the xylary procambium show a separation from the thin cell wall. These stainable pericyclic cells are designated ''.alpha.-cells''. The .alpha.-cells were localized 550 .mu.-2150 .mu. from the apical initials. Three to six pericyclic cells in which the protoplasm scarcely stained with the dye solution are designated ''.beta.-cells''. The triarch .beta.-cell zone was localized in a limited area 550 .mu.-1750 .mu. from the apical initials. Two or three sieve elements were observable inside the .beta.-cells. By applying a combination of the Lillie''s formalin fixation and methyl blue staining, small spherical granules take a deep blue stain in the .beta.-cell cytoplasm. The .beta.-cell which contains these granules were interspersed within a limited area of the root tips.

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